TailorCut Gel Breaker Tubes

$70.00

Fragments PAGE and agarose gel slices into small pieces for easy extraction.

Set of 100

SKU: TC-200 Category:

Description

TailorCut Gel Breaker Tubes fragment gel slices with its patent pending design for easy sample extraction.

Documentation

1) Insert gel cutter tool containing the gel slice into top opening of gel breaker tube.
2) Pulse spin the tube to transfer the gel slice into the gel breaker tube. Discard the gel cutter tool.
3) Insert gel breaker tube containing the gel slice into a 1.5 mL microcentrifuge tube.
4) Centrifuge the tube at 10,000+ xG for 1 minute. Ensure that all gel debris has flown through into the microcentrifuge tube.
5) Add sufficient TE + 0.1% Tween 20 buffer to entirely submerge gel debris.
6) Incubate the tube at room temperature and shake at 1400rpm for a minimum of 2 hours. Overnight incubation is recommended for maximum recovery.
7) Centrifuge the tube at 10,000+ xG for 1 minute.
8) Using a P10 pipette tip, carefully transfer all eluate into a fresh 1.5 mL microcentrifuge tube. The recovered eluate can now be used for downstream processes.

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