Bacterial Genome Sequencing

sequence(1).fastaSeqMatic’s bacterial genome sequencing services provides the highest quality libraries and sequence data for all microbial genomes. The Illumina MiSeq platform can provide high quality sequencing with reads of up to 2x300bp.  We also offer bioinformatic analysis according to the customer’s needs. A wide range of analyses can be conducted including de novo assembly, reference genome mapping, genome annotation, and SNP identification.

Service Highlights:

  • Comprehensive service: We offer DNA extraction, library generation, sequencing, and data analysis services.
  • Prepare libraries from difficult samples: We have experience working with difficult samples such GC rich, and repetitive genomes.
  • High Quality Library Generation: All libraries are size selected to optimize insert size, yielding the highest possible data quality.
  • Stringent Quality Controls: All samples are validated to ensure high quality sequencing data
  • State of the Art Technology: Illumina MiSeq capable of up to 25M reads and read lengths of up to 2x300bp

 

Bacterial Genome Sequencing Service Overview:

DNA Extraction

Library Preparation

Sequencing

Data Analysis

DNA is extracted from bacterial strain. All samples are validated for high yield and purity. Libraries are generated using TruSeq or Nextera protocols and include a unique barcode sequence. All libraries are verified for quality and yield. Sequencing is performed using the Illumina MiSeq. Up to 96 barcoded samples can be pooled into a single run. Sequences can be aligned to reference genomes or assembled into contigs. All raw and processed data is delivered to the customer.

Sample QC Data:

Insert

Insert size plot of SeqMatic generated library.

 

Scaffolds Genome Size Longest Scaffold N50 Raw reads
102 5152810 363308 127936 1067272
De novo assembly performance from 1M reads.

 

Citations & References

Obradović, Davor, et al. “A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160.PloS one 11.7 (2016): e0159231.

Hemp, James, et al. “Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae.Genome Announcements 3.6 (2015): e01353-15.

Ward, Lewis M., et al. “Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae.Genome Announcements 3.6 (2015): e01356-15.

Hemp, James, et al. “Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae.Genome Announcements 3.6 (2015): e01357-15.

Hemp, James, et al. “Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia.Genome Announcements 3.6 (2015): e01347-15.

Pace, Laura A., et al. “Draft Genome of Thermanaerothrix daxensis GNS-1, a Thermophilic Facultative Anaerobe from the Chloroflexi Class Anaerolineae.Genome Announcements 3.6 (2015): e01354-15.

Ward, Lewis M., et al. “Draft Genome Sequence of Herpetosiphon geysericola GC-42, a Nonphototrophic Member of the Chloroflexi Class Chloroflexia.Genome Announcements 3.6 (2015): e01352-15.

Tavormina, Patricia L. et al. “Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the ‘deep sea-1’ clade of marine methanotrophs” Int J Syst Evol Microbiol January 2015 65:251-259; published ahead of print October 23, 2014, doi:10.1099/ijs.0.062927-0

Merga JY, Winstanley C, Williams NJ, Yee E, Miller WG. 2013. Complete genome sequence of the Arcobacter butzleri cattle isolate 7h1h. Genome Announc. 1(4):e00655-13. doi:10.1128/genomeA.00655-13.

Additional Information