Sample Input Recommendations

The following are SeqMatic’s recommendation for sample input for our library generation services. Please consult with your SeqMatic contact for assistance if your samples cannot meet our requirements.

Sample Cells or Tissues:

  • Due to wide range of possible samples types, please discuss with SeqMatic scientist regarding tissue quantity required
  • All samples must be flash frozen or stored in RNA Later

Genomic or Amplicon DNA:

  • Recommended Volume: 20uL or more
  • Recommended Concentration: 20 ng/uL or higher
  • Minimum Volume: 10uL
  • Minimum Concentration: 10 ng/uL
  • A260:A280 value should be between 1.8 and 2.0
  • DNA should be buffered in 10mM Tris-HCl or TE buffer
  • Amplicon DNA should be free of PCR primers and dimers

miRNA and mRNA:

  • We recommend submitting total RNA for miRNA and mRNA projects
  • RNA should be DNA free
  • Recommended Volume: 20uL or more
  • Recommended Concentration: 20 ng/uL or higher
  • Minimum Volume: 10uL
  • Minimum Concentration: 10 ng/uL
  • A260:A280 value should be between 1.8 and 2.0
  • RNA should be stored in nuclease free water or RNA Stable.
  • RNA must validated using an assay such as a BioAnalyzer RNA chip. RNA QC services are available for an additional fee.

Prepared Sequencing Library:

  • Recommended Volume: 20uL or more
  • Recommended Concentration: 10nM or higher
  • Minimum Volume: 10uL
  • Minimum Concentration: 2nM
  • Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
  • Provide at least 10uL of 100uM custom sequencing primers, if required