TailorMix (Non-Denatured) Dual-Indexed PhiX Control Library

$1,794.00$3,348.00

Barcoded PhiX library for high quality NGS data without PhiX contamination.

For monitoring index-swapping in library pools.

Unit Content: 10nM non-denatured library in TE, 10uL

 

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(previous SKU: TM-580)
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TailorMix (Non-Denatured) Dual-Indexed PhiX Control Library

$1,794.00$3,348.00

Description

SeqMatic’s TailorMix Dual-Indexed PhiX control library is an adapter ligated library used as a control for Illumina sequencing instruments. Our custom indexed library eliminates PhiX contamination in multiplexed sequencing runs, enabling the generation of cleaner raw data and the option to analyze the PhiX reads for quality control purposes such as monitor library barcode swapping. Each tube contains 10uL of double stranded library at a concentration of 10nM and can be used as a direct replacement for Illumina PhiX V3.

Features

Positive Control Library for Illumina NGS

  • Compatible with all Illumina NGS systems including NovaSeq 6000, HiSeq 4000, HiSeq 2500, MiSeq and NextSeq
  • Data can be used for sequencing run quality control and index swap monitoring

Read Length (bp)

 

i7 Index i5 index
all Illumina systems MiSeq / HiSeq / NovaSeq MiniSeq / NextSeq /HiSeq
10 bp TCGAATGATC GACATGCGAC GTCGCATGTC

⇒ Download User Guide (TM-581 & TM-582-UG): TailorMix Dual-Indexed PhiX Control Library (Non-denatured)

 

High Flexibility

  • Positive control library used to monitor instrument error rates and read quality
  • High diversity library can be used to supplement low nucleotide diversity sequencing runs

Unique Dual-Index Barcodes

  • Custom designed dual-index sequences (can also be used in single index runs)
  • Compatible with all SeqMatic and Illumina sample prep kits
  • Additional barcode combinations available as custom orders

Easy to Use

  • Supplied as a double stranded 10nM stock in TE
  • Direct replacement for Illumina PhiX V3

Recommended For:

  • 16S amplicon sequencing
  • Single PCR amplicon sequencing
  • Viral genome sequencing
  • Low diversity libraries
  • Highly multiplexed pools
  • Single sample runs
  • Unindexed libraries

Specifications

Package Size Pack of 6, Pack of 12

 

 

Sample Data

Figure 1: Percentage of sequencing reads mapping to the PhiX 174 genome using Indexed PhiX compared against Illumina PhiX V3.

 

Additional

Weight N/A
Dimensions N/A
Size

Pack of 6, Pack of 12

Customer Testimonials

“PhiX contamination in our data used to be a significant issue given that we were sequencing viral DNA extracted from patient biopsies.  We no longer see PhiX contamination with your product, which ultimately streamlines our data processing and gives us additional peace-of-mind regarding the validity of our data.”

Eric Sherman
University of Pennsylvania

“My lab was noticing large amounts of bleed-through of Illumina’s PhiX v3 into our indexed samples. Seqmatic’s Indexed PhiX dramatically reduces this bleed-through and allows us to estimate our incidence of sequencing crossover contamination.”

Customer C.F.
San Francisco, CA

Citations & References

Shechner, David M., et al. “Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.” Nature methods 12.7 (2015): 664-670. 

Get Your Results

Optimal solutions are custom-made according to research needs

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Pre-Submission

Planning & Consultation - Assurance of de-identified sample submission and handling

Sample Submission

Nucleic Acid Extraction - We accept a wide range of sample types

Library Preparation

Library Generation - Amplified cDNA is used for sequence libraries' construction

Sequencing

Different platforms for different data outputs

Data Analysis

Custom BioInformatics