Description
SeqMatic’s TailorMix Dual-Indexed PhiX control library is an adapter ligated library used as a control for Illumina sequencing instruments. Our custom indexed library eliminates PhiX contamination in multiplexed sequencing runs, enabling the generation of cleaner raw data and the option to analyze the PhiX reads for quality control purposes such as monitor library barcode swapping. Each tube contains 10uL of double stranded library at a concentration of 10nM and can be used as a direct replacement for Illumina PhiX V3.
Features
Positive Control Library for Illumina NGS
- Compatible with all Illumina NGS systems including NovaSeq 6000, HiSeq 4000, HiSeq 2500, MiSeq and NextSeq
- Data can be used for sequencing run quality control and index swap monitoring
Read Length (bp)
| i7 Index | i5 index | ||
|---|---|---|---|
| all Illumina systems | MiSeq / HiSeq / NovaSeq | MiniSeq / NextSeq /HiSeq | |
| 10 bp | TCGAATGATC | GACATGCGAC | GTCGCATGTC |
⇒ Download User Guide (TM-581 & TM-582-UG): TailorMix Dual-Indexed PhiX Control Library (Non-denatured)
High Flexibility
- Positive control library used to monitor instrument error rates and read quality
- High diversity library can be used to supplement low nucleotide diversity sequencing runs
Unique Dual-Index Barcodes
- Custom designed dual-index sequences (can also be used in single index runs)
- Compatible with all SeqMatic and Illumina sample prep kits
- Additional barcode combinations available as custom orders
Easy to Use
- Supplied as a double stranded 10nM stock in TE
- Direct replacement for Illumina PhiX V3
Recommended For:
- 16S amplicon sequencing
- Single PCR amplicon sequencing
- Viral genome sequencing
- Low diversity libraries
- Highly multiplexed pools
- Single sample runs
- Unindexed libraries
Specifications
| Package Size | Pack of 6, Pack of 12 |
|---|
Sample Data
Figure 1: Percentage of sequencing reads mapping to the PhiX 174 genome using Indexed PhiX compared against Illumina PhiX V3.
