Description
SeqMatic’s TailorMix dual-indexed PhiX control library is a adapter ligated library used as a control for Illumina sequencing instruments. Our custom indexed library eliminates PhiX contamination in multiplexed sequencing runs, enabling the generation of cleaner raw data and the option to analyze the PhiX reads for quality control purposes. Each tube contains 200uL of single stranded library prediluted in HT1 buffer to a concentration of 20pM, ready for spike in.
⇒ NOTE: Non-denatured PhiX is available for platforms using ExAmp cluster generation (NovaSeq, HiSeq X and HiSeq 3000/4000). Denatured PhiX is not compatible with those platforms.
Features
Positive Control Library for Illumina NGS
- Compatible with most Illumina NGS systems including HiSeq 2500, MiSeq, and NextSeq
- Note: Use non-denatured PhiX for NovaSeq HiSeq X and HiSeq 3000/4000
Read Length (bp)
| i7 Index | i5 index | ||
|---|---|---|---|
| all Illumina systems | MiSeq / HiSeq / NovaSeq | MiniSeq / NextSeq /HiSeq | |
| 10 bp | TCGAATGATC | GACATGCGAC | GTCGCATGTC |
⇒ Download User Guide (TM-503 & TM-504): TailorMix Dual-Indexed PhiX Control Library (Denatured)
High Flexibility
- Positive control library used to monitor instrument error rates and read quality
- High diversity library can be used to supplement low nucleotide diversity sequencing runs
Unique Dual-Index Barcodes
- Custom designed dual-index sequences (can also be used in single index runs)
- Compatible with all SeqMatic and Illumina sample prep kits
Easy to use
- Supplied as a single stranded 20pM stock in HT1 buffer
- Library ready for immediate spike in
Recommended For:
- 16S amplicon sequencing
- Single PCR amplicon sequencing
- Viral genome sequencing
- Low diversity libraries
- Highly multiplexed pools
- Single sample runs
- Unindexed libraries
Specifications
| Package Size | Pack of 6, Pack of 12 |
|---|
Sample Data
Figure 1: Percentage of sequencing reads mapping to the PhiX 174 genome using Indexed PhiX compared against Illumina PhiX V3.
