TailorMix (Denatured) Dual-Indexed PhiX Control Library

Barcoded PhiX library for high quality NGS data without PhiX contamination.

Unit Content:  20pM denatured library in HT1 buffer, 200uL

 

N/A

$1,794.00$3,348.00

TailorMix (Denatured) Dual-Indexed PhiX Control Library

$1,794.00$3,348.00

Description

SeqMatic’s TailorMix dual-indexed PhiX control library is a adapter ligated library used as a control for Illumina sequencing instruments. Our custom indexed library eliminates PhiX contamination in multiplexed sequencing runs, enabling the generation of cleaner raw data and the option to analyze the PhiX reads for quality control purposes. Each tube contains 200uL of single stranded library prediluted in HT1 buffer to a concentration of 20pM, ready for spike in.

NOTE: Non-denatured PhiX is available for platforms using ExAmp cluster generation (NovaSeq, HiSeq X and HiSeq 3000/4000). Denatured PhiX is not compatible with those platforms.

Features

Positive Control Library for Illumina NGS

  • Compatible with most Illumina NGS systems including HiSeq 2500, MiSeq, and NextSeq
  • Note: Use non-denatured PhiX for NovaSeq HiSeq X and HiSeq 3000/4000

Read Length (bp)

i7 Index i5 index
all Illumina systems MiSeq / HiSeq / NovaSeq MiniSeq / NextSeq /HiSeq
10 bp TCGAATGATC GACATGCGAC GTCGCATGTC

⇒ Download User Guide (TM-503 & TM-504): TailorMix Dual-Indexed PhiX Control Library (Denatured)

 

High Flexibility

  • Positive control library used to monitor instrument error rates and read quality
  • High diversity library can be used to supplement low nucleotide diversity sequencing runs

Unique Dual-Index Barcodes

  • Custom designed dual-index sequences (can also be used in single index runs)
  • Compatible with all SeqMatic and Illumina sample prep kits

Easy to use

  • Supplied as a single stranded 20pM stock in HT1 buffer
  • Library ready for immediate spike in

Recommended For:

  • 16S amplicon sequencing
  • Single PCR amplicon sequencing
  • Viral genome sequencing
  • Low diversity libraries
  • Highly multiplexed pools
  • Single sample runs
  • Unindexed libraries

Specifications

Package Size Pack of 6, Pack of 12

Sample Data

 

Figure 1: Percentage of sequencing reads mapping to the PhiX 174 genome using Indexed PhiX compared against Illumina PhiX V3.

Additional

Weight N/A
Dimensions N/A
Kit Size

Pack of 6, Pack of 12

Customer Testimonials

“PhiX contamination in our data used to be a significant issue given that we were sequencing viral DNA extracted from patient biopsies.  We no longer see PhiX contamination with your product, which ultimately streamlines our data processing and gives us additional peace-of-mind regarding the validity of our data.”

Eric Sherman
University of Pennsylvania

 

“My lab was noticing large amounts of bleed-through of Illumina’s PhiX v3 into our indexed samples. Seqmatic’s Indexed PhiX dramatically reduces this bleed-through and allows us to estimate our incidence of sequencing crossover contamination.”

Customer C.F.
San Francisco, CA

Citations & References

Shechner, David M., et al. “Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.” Nature methods 12.7 (2015): 664-670.

Get Your Results

Optimal solutions are custom-made according to research needs

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Pre-Submission

Planning & Consultation - Assurance of de-identified sample submission and handling

Sample Submission

Nucleic Acid Extraction - We accept a wide range of sample types

Library Preparation

Library Generation - Amplified cDNA is used for sequence libraries' construction

Sequencing

Different platforms for different data outputs

Data Analysis

Custom BioInformatics