SeqMatic’s TailorMix dual-indexed PhiX control library is a adapter ligated library used as a control for Illumina sequencing instruments. Our custom indexed library eliminates PhiX contamination in multiplexed sequencing runs, enabling the generation of cleaner raw data and the option to analyze the PhiX reads for quality control purposes. Each tube contains 200uL of single stranded library prediluted in HT1 buffer to a concentration of 20pM, ready for spike in.
Note: Non-denatured PhiX is available for platforms using ExAmp cluster generation (NovaSeq, HiSeq X and HiSeq 3000/4000). Denatured PhiX is not compatible with those platforms.
Positive Control Library for Illumina NGS
- Compatible with most Illumina NGS systems including HiSeq 2500, MiSeq, and NextSeq
- Note: Use non-denatured PhiX for NovaSeq HiSeq X and HiSeq 3000/4000
- Positive control library used to monitor instrument error rates and read quality
- High diversity library can be used to supplement low nucleotide diversity sequencing runs
Unique Dual-Index Barcodes
- Custom designed dual-index sequences (can also be used in single index runs)
- Compatible with all SeqMatic and Illumina sample prep kits
Easy to use
- Supplied as a single stranded 20pM stock in HT1 buffer
- Library ready for immediate spike in
- 16S amplicon sequencing
- Single PCR amplicon sequencing
- Viral genome sequencing
- Low diversity libraries
- Highly multiplexed pools
- Single sample runs
- Unindexed libraries
Figure 1: Percentage of sequencing reads mapping to the PhiX 174 genome using Indexed PhiX compared against Illumina PhiX V3.
“PhiX contamination in our data used to be a significant issue given that we were sequencing viral DNA extracted from patient biopsies. We no longer see PhiX contamination with your product, which ultimately streamlines our data processing and gives us additional peace-of-mind regarding the validity of our data.”
University of Pennsylvania
“My lab was noticing large amounts of bleed-through of Illumina’s PhiX v3 into our indexed samples. Seqmatic’s Indexed PhiX dramatically reduces this bleed-through and allows us to estimate our incidence of sequencing crossover contamination.”
San Francisco, CA
Citations & References:
Shechner, David M., et al. “Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.” Nature methods 12.7 (2015): 664-670.