
Bulk ATAC-Seq Analysis Services
Gene regulation and chromatin structure across the genome
Bulk ATAC-seq is a versatile technique that has contributed significantly to the understanding of gene regulation and chromatin structure across the genome. SeqMatic offers cost-efficient bulk ATAC-seq analysis services for comparative studies of chromatin states between conditions; gene regulation for changing chromatin accessibility during development; and also, for identification of biomarkers and therapeutic oncology targets.
Due to the versatility of this technique, it is noteworthy to mention that the use of bulk ATAC-seq analysis services versus single cell ATAC-seq services depends on several factors, including your experimental design, the biological question you want to resolve, and your assigned budget considerations.
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Workflow for Bulk ATAC-seq Analysis:
(A) Schematic of Library preparation
(B) Visual of transposition results in fragmented DNA. Adapters have to be completed with a 72°C extension step prior to amplification. Then, during PCR, additional sequence is incorporated into the adapters (including common sequencing ends and a sequencing barcode).
Bulk ATAC-seq Applications
At SeqMatic, bioinformatic analysis services of bulk ATAC-seq applications include:
- Nucleosome mapping for identification of nucleosome positions and occupancy across the genome.
- Transcription factor binding analysis to enable the identification of transcription factor binding sites and the study of their role in gene regulation.
- Novel enhancer identification to study enhancer’s role in gene expression.
- Exploration of disease-relevant regulatory mechanisms to understand the role of chromatin accessibility in complex diseases.
- Cell type-specific regulation analysis to provide insights into cell type-specific regulatory mechanisms.
- Evolutionary studies to understand the evolution of chromatin accessibility across different species.
- Comparative epigenomics to compare profiles between different cell types or conditions.
- Biomarker discovery to identify potential biomarkers associated with specific diseases or conditions
If your research goal is to get insights about cell lines or tissues composed of a single cell type, bulk ATAC-seq is absolutely a good choice. Also, it helps measure chromatin accessibility in cell populations with a high degree of homogeneity.
We can process full workflows or accept your tissue, cells, fresh/frozen nuclei for library preparation or your prepared libraries for sequencing.
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SeqMatic’s Premium Collaboration ServicesFetch-Seq courier pick-up service in the S.F. Bay Area |
- Elizaldi, Sonny R., et al. “CCR7+ CD4 T Cell Immunosurveillance Disrupted in Chronic SIV-Induced Neuroinflammation in Rhesus Brain.” bioRxiv (2023): 2023-08.
- Mich, John K., et al. “Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex.” Cell reports 34.13 (2021).
- Cambier, Linda, et al. “Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers.” Scientific Reports 11.1 (2021): 94.
- Rajan, Saravanan, et al. “Recombinant human B cell repertoires enable screening for rare, specific, and natively paired antibodies.” Communications Biology 1.1 (2018): 5.
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