Bulk mRNA Sequencing Services
Quantifying Gene Expression Accurately
Bulk RNA-Seq is the leading technique in the quest to unravel the intricate molecular mechanisms behind cell activity at a moment in time. The SeqMatic bulk mRNA sequencing services support a number of research projects that hone in on differentially expressed genes, offering valuable insights into the molecular processes involved in various stages of cell growth, tumorigenesis, immune response, etc.
Our scientist-to-scientist consultative approach is key to enable the best result for your scientific investigation. We learn about the goals of our customer’s scientific research, then we modify our RNA workflows and kit selection to target those specific goals — all coupled with world class turnaround times. The application of best science, higher quality data, superior consistency, and fast delivery makes SeqMatic an exceptional research partner.
Stringent Quality Controls – SeqMatic qualifies RNA with a TapeStation Electropherogram for each RNA sample submitted. TapeStation analysis includes RNA Integrity Number (RIN) and DV200 for each sample. Results are shared for review and approval prior to proceeding to library preparation.
Scientific considerations for read depth – SeqMatic recommends a read depth of 25-30M reads per sample for standard transcriptome analysis. Higher and lower read depths are available depending on model organism requirements and/or project needs.
Preliminary Sequencing – After library preparation, SeqMatic prepared libraries are pooled to customer requirements and the final QC is checked via preliminary sequencing (MiSeq). Libraries are then normalized and re-pooled based on the read count from the preliminary sequencing dataset.
World-class validation standard & practices – SeqMatic is equipped to ensure accurate, repeatable and reproducible results. SeqMatic’s quality program has been audited and approved as part of becoming a Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) certified sequencing facility. Our CLIA quality program requires the use of well-defined protocols to maintain the consistency and reproducibility of our NGS workflows. Thus, the samples will be processed in a CLIA environment.
Custom assay services – Our highly personalized sequencing services can be adapted to almost any (high and/or low throughput) sequencing workflow required by our customers so that we optimize the quality and quantity of data output.
Comprehensive Services
RNA workflow services include extraction from virtually any source, library generation, sequencing, and a full suite of bioinformatics support.
Expert Volume Handling
From small projects with a few samples or low input to large scale high-throughput projects
Prepared Libraries From Difficult Samples
We have experience working with low input, archival, non-model organisms, FFPE, etc.
Wide Range of Sample Types
Human, animal, plant, fish, bacteria, yeast, viruses, environmental material, etc.
Bioinformatics
- Full suite of custom analytic pipelines available for individual project needs, including support for non-model organisms
- Comprehensive differential expression report available as part of SeqMatic’s standard bioinformatics pipeline
Sequencing Platforms
Sequencing on MiSeq, NextSeq, NovaSeq 6000 and NovaSeq X Plus
SeqMatic’s strand-specific bulk mRNA sequencing services empower researchers to uncover both established and newfound aspects of gene expression. This analysis boasts a myriad of applications, ranging from cancer research (including strategies for prevention and treatment) to disease-specific gene expression studies and RNA drug response biomarker investigations, making it an indispensable tool in the realm of modern scientific exploration.
- Naphade, Swati, et al. “Systematic comparative analysis of strand-specific RNA-seq library preparation methods for low input samples” Scientific Reports 12, Article number: 1789 (2022)
- Pecman, Anja, et al. “Next Generation Sequencing for Detection and Discovery of Plant Viruses and Viroids: Comparison of Two Approaches” Front. Microbiol., 13 October 2017
- M. Eicholtz, S. Grinstead, L.-P. Wu, G. Kinard, and R. Li “First Report of Beet western yellows virus Infecting Epiphyllum spp” APS Publications Published Online:12 Dec 2017
Find other Research Publications from around the world!