Visual illustration of floating bulk total RNA

Bulk Total RNA & Non-coding RNA Services

Gene expression preferred methods for transcriptomic analysis

Bulk total RNA & non-coding RNA services are a great option for a cost-effective, comprehensive overview of gene expression levels in a sample. Bulk Total RNA with RiboZero+ sequencing is the preferred method for transcriptomic analysis of whole blood, cells (i.e. bacteria) and tissues (i.e. spleen) where mRNA with PolyA tails are not available or overwhelmed by globin RNAs. It is often used for poor quality samples (FFPE, etc.). Total RNA with RiboZero+ preparations enrich for mRNAs via structural RNA depletion. Bulk RNA sequencing can measure the average expression level of individual genes across a population of cells to provide a global idea of gene expression, even differences between difficult or poor-quality samples.

SeqMatic’s Total RNA library preparation and sequencing can detect coding plus multiple forms of noncoding RNA. Total RNA-Seq can identify structural RNAs including non-coding, small and miRNAs. SeqMatic’s Total RNA-Seq solutions provide optimal coverage of normal or low-quality samples.

Non-coding RNA (ncRNAs) sequencing contributes to the control of both normal and disease-related cellular processes by modulating both gene expression and regulation.

Long non-coding RNAs (lncRNAs) are abundant within the eukaryotic transcriptome and comprised of longer than 200nt non-coding RNAs. lncRNAs affect multiple cellular functions through their regulation of gene transcription, post-transcriptional modifications, and epigenetics. At SeqMatic, non-coding RNA sequencing research studies analyze functional roles in diverse biological processes and human diseases, such as neurological disorders and cancers.

HIDDEN
  • Tissues
  • Cells
  • Exosomes
  • FFPE
  • Whole Blood
  • Serum
  • Environmental

Service Portfolio

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TailorMix Dual-Indexed PhiX