Bulk Total RNA & Non-coding RNA Services
Gene expression preferred methods for transcriptomic analysis
Bulk total RNA & non-coding RNA services are a great option for a cost-effective, comprehensive overview of gene expression levels in a sample. Bulk Total RNA with RiboZero+ sequencing is the preferred method for transcriptomic analysis of whole blood, cells (i.e. bacteria) and tissues (i.e. spleen) where mRNA with PolyA tails are not available or overwhelmed by globin RNAs. It is often used for poor quality samples (FFPE, etc.). Total RNA with RiboZero+ preparations enrich for mRNAs via structural RNA depletion. Bulk RNA sequencing can measure the average expression level of individual genes across a population of cells to provide a global idea of gene expression, even differences between difficult or poor-quality samples.
SeqMatic’s Total RNA library preparation and sequencing can detect coding plus multiple forms of noncoding RNA. Total RNA-Seq can identify structural RNAs including non-coding, small and miRNAs. SeqMatic’s Total RNA-Seq solutions provide optimal coverage of normal or low-quality samples.
Non-coding RNA (ncRNAs) sequencing contributes to the control of both normal and disease-related cellular processes by modulating both gene expression and regulation.
Long non-coding RNAs (lncRNAs) are abundant within the eukaryotic transcriptome and comprised of longer than 200nt non-coding RNAs. lncRNAs affect multiple cellular functions through their regulation of gene transcription, post-transcriptional modifications, and epigenetics. At SeqMatic, non-coding RNA sequencing research studies analyze functional roles in diverse biological processes and human diseases, such as neurological disorders and cancers.
- Tissues
- Cells
- Exosomes
- FFPE
- Whole Blood
- Serum
- Environmental
Consultative Support – We can help you determine the best choice of RNA-seq solutions for your sample type and research goals
Comprehensive Services – Complete RNA workflow, including extraction from virtually any sample type, library generation, sequencing, and data analysis services
Prepared Libraries From Difficult Samples – We have experience working with poor quality, low input, archival, non-model organisms, FFPE, plasma, serum, biopsies, etc.
Stringent Quality Controls – All sample preparation and library construction steps are validated by multiple assays (TapeStation, qPCR and test sequencing). QC reports prepared for review by customer to decide which samples qualify for library preparation, or alternative library preparation/sample re-submission
Bioinformatics – Full suite of pipelines available, as well as custom solutions for your transcriptome and pathway analysis. Check our Bioinformatics page and sample report for more information.
Fast Turnaround Time – Standard and Express Services available
Flexibility – From small projects with low input to large scale projects with high-throughput. Manually or automated processing using a wide-range of technologies
Wide-range of Sample Types – virtually any source: human, animal, fish, plant, bacteria/fungi, environmental, viruses/phages
Expert Pooling Validated by Test Sequencing – to ensure equal sample sequencing coverage
Library Preparation – Illumina Total RNA with RZ+ is our standard offering, however we can use alternate library preparation kits as specified by customer
Scalability from Pilot to Production – SeqMatic is your one partner for any scale project. No sample minimum or maximum!
All RNA projects are performed in a CLIA environment – To ensure consistent quality and reproducibility
- Gavrili, Vasileia, et al. “First report of barley virus G infecting corn in Greece.” Journal of Plant Pathology 103 (2021): 1331-1331.
- Mehle, N., et al. “First report of cucurbit aphid-borne yellows virus in Cucurbita pepo and Cucurbita maxima in Slovenia.” Plant Disease 104.2 (2020): 599-599.
- Wintermantel, W. M., et al. “First report of Cucurbit chlorotic yellows virus infecting melon in the New World.” Plant Disease 103.4 (2019): 778-778.
- Pollet, Kato, et al. “Host miRNAs as biomarkers of SARS-CoV-2 infection: a critical review.” Sensors & Diagnostics 2.1 (2023): 12-35.
- Martínez-González, Elena, et al. “Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients.” Scientific Reports 10.1 (2020): 11140.
- Van Goethem, Alan, et al. “Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples.” Scientific Reports 6.1 (2016): 37876.
Find other Research Publications from around the world!