Epigenome Profile Sequencing Services
HIGH-THROUGHPUT GENE EXPRESSION PROFILING & REGULATION
Epigenome profile sequencing services are important for understanding gene regulation. At SeqMatic, we offer a variety of epigenome sequencing profiling services to generate data for analysis of epigenetic modifications, and the impact on gene regulation. It is important to highlight, that our laboratory employs Illumina NovaSeq platforms for high throughput epigenomic profiling.
Comprehensive Analysis Services for Epigenome Profiling
Our laboratory supports research studies across diverse genome, transcriptome and epigenome applications for many organisms. And our profiling services are mostly engaged by research studies that investigate the various aspects of chromatin architecture.
Moreover, the SeqMatic laboratory offers epigenomic analysis methods that include: DNA methylation (bisulfite sequencing); Single-cell ATAC-Seq and Bulk ATAC-Seq for chromatin accessibility; and ChIP-Seq for examining transcription factor binding. In addition to these, our laboratory also offers Multiomics for integrated ATAC-Seq and gene expression analyses.
SeqMatic Service Solutions for Epigenome Profiling:
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Bulk ATAC-Seq
SeqMatic offers Bulk ATAC-Seq services for efficient detection of chromatin accessibility and its impact on gene regulation, cell fate determination, and disease processes. The Assay for Transposase-Accessible Chromatin with high-throughput sequencing utilizes a hyperactive Tn5 transposase method, which allows for bulk processing. The Tn5 transposase is used to insert sequencing adapters into open chromatin regions. By capturing temporal changes in chromatin accessibility, ATAC-Seq provides valuable insights into the underlying molecular mechanisms that drive biological processes and disease progression.
When analyzing changes over time in ATAC-seq data, a primary focus is on identifying the open chromatin regions (OCRs) that undergo changes throughout the process. In this context, the input for analysis consists of the differential OCRs between each pair of time points. Unlike pseudotime analysis in single cells, bulk ATAC-seq data is generated at predetermined time points. This eliminates the need to infer the temporal order between samples.
> Learn about SeqMatic’s Bulk ATAC-Seq services
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Methylation (Methyl-Seq)
DNA methylation is an important mechanism in the field of epigenetics. This process involves the addition of a methyl group to a molecule, leading to changes that can influence the expression of certain genes and impact biological functions. Gene expression research studies engage SeqMatic methylation services for genome-wide analysis as well as targeted approaches.
SeqMatic’s high-throughput targeted bisulfite sequencing (Methyl-Seq) solutions enable the evaluation of site-specific DNA methylation changes and the identification of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) within genomes.
The SeqMatic comprehensive methylation analyses provide valuable insights into methylation patterns at a single nucleotide level for a number of applications including the study of disease states such as cancer, monitoring embryonic development, and advancing research in the field of agricultural plants.
Learn more about SeqMatic’s Methylation services (Methyl-Seq)
EM-seq and sodium bisulfite conversion methods
Figure Source: New England BioLabs Inc. Technical Note “NEBNext® Enzymatic Methyl-seq (EM-seq™)”
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Single Cell ATAC-Seq
The simplicity and scalability provided by the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) drives the popularity and adoption of this technique for the detection and assessment of genome-wide chromatin accessibility of cell types and how their normal state may be altered by triggers.
> Learn more about SeqMatic’s ATAC-Seq services
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Chromatin Immunoprecipitation (ChIP-Seq)
ChIP-Seq is a powerful method that combines chromatin immunoprecipitation with Next-Generation Sequencing (NGS) to conduct comprehensive surveys of gene regulation on a genome-wide scale.
The SeqMatic laboratory offers ChIP-Seq as a valuable tool to investigate protein interactions with DNA and the binding sites of transcription factors. This technique is particularly useful in research projects focused on understanding the progression of cancer and other diseases. By generating millions of counts across multiple samples, ChIP-Seq yields important data and insights into gene regulation.
ChIP-Seq Advantages For Epigenome Profile Sequencing:
One of the key advantages of the ChIP-Seq method is its compatibility with various types of input DNA samples. It enables the identification of DNA targets bound by transcription factors and histone modifications throughout an organism’s entire genome. Moreover, when combined with RNA sequencing and methylation analysis, ChIP-Seq unveils intricate gene regulation networks. This approach provides a comprehensive understanding of how transcription factors interact with DNA and the impact on gene expression.
Workflow Image Source: Wikipedia free licensed image
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10x Genomics Chromium Multiome
10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression – The SeqMatic facility offers the ability to simultaneously profile gene expression and open chromatin within the same cell, thereby enabling a more comprehensive characterization of cell types and states through dual readouts. This integrated method proves especially valuable in discerning disease subtypes and predicting biomarkers. The multidimensional aspect of the multi-omics solution supports numerous research projects with focus on cancer and other diseases, including the investigation of genetic and phenotypic alterations, as well as resistance mechanisms to target therapies.
> Learn about SeqMatic’s Single Cell ATAC-Seq Services
> Learn about SeqMatic’s Single Cell Gene Expression Services
Service Features
Epigenome Profile Sequencing Service Highlights
Service Features
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Epigenetic Discovery – Expression and Regulation |
Epigenome Profile Sequencing Service Highlights
 SeqMatic Onsite-Seq™ Premium Mobile Service Operations |
 SeqMatic Fetch-Seq™ Premium Courier Service Operations |
| We can come to your laboratory! We provide “Onsite-Seq” service for all Single Cell RNA sequencing needs. | Simplify handling and transportation of your samples. Your domestic advantage available to the S.F. Bay Area. |
Epigenome Profile Sequencing Service Highlights
- Expert Consultation – Our scientists can help you select the most scientifically viable and cost-effective choice of Single-Cell or Bulk ATAC-Seq and/or Methyl-Seq
- Comprehensive Services – Complete DNA workflow, including extraction from virtually any sample type, library generation, sequencing, and data analysis services
- Prepare Libraries From Difficult Samples – Low input DNA (as low as 1ng), blood, blood cards, saliva, stool, cell-free DNA and FFPE
- Stringent Quality Controls – All sample preparation and library construction steps are validated by multiple assays (Qubit, TapeStation, qPCR and test sequencing)
- Fast Turnaround Time – Standard and Express Services available
- Technical Support – QC reports prepared for review by the customer to decide which samples qualify for library preparation, or alternative library preparation/sample re-submission
- Flexibility – Choice for sample processing: manually or automated using a wide-range of processing technologies
- Wide-Range of Sample Types – human, animal, fish, from virtually any source
DISCLAIMER: Supporting images (charts, figures, tables) and descriptions on this page are adapted from information originally compiled and presented by the various sources which are cited and linked within each individual topic module.
The SeqMatic Difference
Expert NGS
The SeqMatic approach starts with proven genomic workflows and proprietary controls that have been refined and optimized over the past decade to deliver considerably more data than Illumina specifications.
Expert NGS
The SeqMatic approach starts with proven genomic workflows and proprietary controls that have been refined and optimized over the past decade to deliver considerably more data than Illumina specifications.
View MoreConscientious Attention To Detail
We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.
Conscientious Attention To Detail
We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.
View MoreTailor-made Services with Fast TAT
Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.
Tailor-made Services with Fast TAT
Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.
View MoreOptimal Solutions to Research Needs
This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.
Optimal Solutions to Research Needs
This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.
View MoreGet Your Results
Optimal solutions are custom-made according to research needs
Pre-Submission
Planning & Consultation - Assurance of de-identified sample submission and handling
Sample Submission
Nucleic Acid Extraction - We accept a wide range of sample types
Library Preparation
Library Generation - Amplified cDNA is used for sequence libraries' construction
Sequencing
Different platforms for different data outputs
Data Analysis
Custom BioInformatics