Methylation (Methyl-Seq) Analysis Services
Enzymatic Methylation - insights at a single nucleotide level
Methylome sequencing analysis services offer crucial understandings of the intricate processes behind gene control, and how these impact wellness, illness, and the ability to adapt to environmental changes.
SeqMatic offers NEBNext® Enzymatic Methyl-seq (EM-seq) services for identification of 5-methylcytosine (5nC) and 5-hydroxymethylcytosine (5hmC) within genomes. This high-performance methylome analyses method provides high-quality libraries with less fragmentation, less biases and minimal loss or damage to DNA when compared to bisulfite methylome analysis using Illumina sequencing.
Methyl-seq has several advantages, including the ability to capture full sample diversity with small amounts of DNA and covers emerging regions of interest in the human genome. At SeqMatic, Methyl-seq applications include studies of disease states (i.e. cancer); monitoring of embryonic development; studies of agricultural plants, etc.
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Enzymatic Methlylation Advantages:
- Superior sensitivity of detection of 5-mC and 5-hmC
- Greater mapping efficiency
- More uniform GC coverage
- Detection of more CpGs with fewer sequence reads
- Uniform dinucleotide distribution
- High-efficiency library preparation, with larger library insert sizes
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Sample Requirements:
200 ng input genomic DNA suggested for best results A minimum of 10ng input is required per sample |
We can process full workflows or accept your tissue, fresh/frozen cells/nuclei, or prepared libraries. |
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 | Your S.F. Bay Area Advantage! To maintain maximum sample viability, your samples can be transported to us |
  | SeqMatic’s Premium Services Onsite-SeqTM brings our experts to your lab to generate the GEMS. Fetch-SeqTM courier pick-up in the S.F. Bay Area |
Schupp, Patrick Georg. Clarifying the Transcriptional Profiles of Malignant Clones and Nonmalignant Cells of the Microenvironment through Multiscale and Multiomic Analysis of Individual Tumors. Diss. UCSF, 2023.
Barker, Scarlett J., et al. “Targeting Transferrin Receptor to Transport Antisense Oligonucleotides Across the Blood-Brain Barrier.” bioRxiv (2023): 2023-04.
Nair, Anup K., et al. “Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians.” Cells 11.9 (2022): 1446.
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