Single Cell Parse Evercode services - SeqMatic is a Certified Service Provider for Parse Biosciences

Single Cell/Nuclei RNA Sequencing Services

Parse Biosciences Certified Service Provider

Single Cell Parse Evercode services provide highly scalable and flexible analysis of individual cells within a tissue sample. SeqMatic is a Certified Service Provider for Parse Biosciences and their Evercode™ technology.

Evercode technology enhances single-cell RNA sequencing (scRNA-seq) and single-nuclei RNA sequencing (snRNA-seq) by enabling the detection of more genes per cell. Evercode™ uses split-pool combinatorial barcoding, which doesn’t require individual cell isolation. In other words, Evercode™ uses the cell or nucleus as the reaction compartment, eliminating limitations with hardware-based solutions.

Single Cell Parse Evercode Diagram

Cells are first fixed and permeabilized, turning them into their own reaction vessels, removing the need to capture individual cells in droplets or microwells. The split-pool barcoding process then labels cells with an exponentially large number of barcode combinations making it possible to easily scale beyond other technologies. Up to 1 million cells, collected on different days, can be processed in parallel.

HIDDEN

Innovative fixation process enables storage up to 6 months, enabling batching of samples for large projects such as time course and / or dosing studies.Batching samples together also reduces the cost per sample and minimizes potential batch to batch variations (batch effects). Eliminate potential biases by processing samples together regardless of when they were collected. Ensure consistency in data analysis and maximize comparability between samples.

Whole Transcriptome for any species

  • Mini kit – Up to 10K cells / 1-12 samples
  • Standard kit – Up to 100K cells / 1-48 samples
  • Mega kit – Up to 1M cells / 1-96 samples

CRISPR gRNA Detection

Single cell pooled CRISPR screens enable analysis of complex phenotypes by linking individual gene perturbations with gene expression profiles. This approach has been used for target validation in drug discovery, to understand differences in treatment responses, and map pathways involved in cell differentiation. Wider adoption of this approach has been constrained by the feasibility and high cost of processing sufficient cells for libraries containing more than a few hundred guide RNAs (sgRNAs). CRISPR Detect brings the scalability of Evercode™ technology to single cell CRISPR screens, making genome-wide studies practical.


T Cell Receptor (TCR) for mouse and human

Capture paired TCRs and gene expression from the same cell Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells. B Cell Receptor (BCR) for mouse and human Capture full length BCR sequences and gene expression together at unprecedented scale Explore a wide variety of adaptive immunity applications through Evercode™ BCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired heavy-light chains to characterize the immune repertoire complexity.

Service Portfolio

PRODUCT HIGHLIGHT

TailorMix Dual-Indexed PhiX