PCR Amplicons & Plasmid Sequencing
Sequence dozens to hundreds of PCR amplicons at once from any source
Amplicon sequencing enables the profiling of PCR products with ultra-high read-depth and high-throughput.
PCR Amplicon Sequencing
- Library Preparation services available
- Long amplicons can be sequenced at full length using MiSeq 500 and 600 cycle kits and the NovaSeq SP500
- NovaSeq 6000 and NovaSeq X are available for high-throughput projects (up to 384 unique samples)
- TailorMixTM Indexed PhiX controls to address low diversity
- Consultation for staggered sequencing to maximize diversity by request
- Your validated custom primers can be used for two-step PCR or during sequencing
Fast Turnaround Time
- 3 to 5 business days Express Service for prepared libraries
- 10 business days Express Service for library preparation
- Pooling and Normalization of pre-made libraries are available
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Stringent Quality Controls – SeqMatic qualifies DNA with a TapeStation Electropherogram and Qubit for each total DNA sample submitted. A TapeStation analysis includes a DNA Integrity Number (DIN) for each sample, where a value of 10 indicates a perfectly intact sample and a value of 1 indicates a severely degraded sample. Results are shared for review and approval prior to proceeding to library preparation.
High-Quality design considerations for read depth – SeqMatic offers typical read depth of 50K to 100K or more reads per sample. Higher and lower read depths are available depending on project needs.
Preliminary Sequencing – After library preparation, libraries are pooled in equal volumes and the final pool is quantified by Agilent TapeStation and checked via preliminary sequencing (MiSeq). Libraries are then normalized and re-pooled based on the read counts from the preliminary sequencing dataset.
World-class validation standard & practices – SeqMatic is equipped to ensure accurate, repeatable and reproducible results. SeqMatic’s quality program has been audited and approved as part of becoming a Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) certified sequencing facility. Our CLIA quality program requires the use of well-defined protocols to maintain the consistency and reproducibility of our NGS workflows. Thus, the samples will be processed in a CLIA environment.
Custom assay services – Our highly personalized sequencing services can be adapted to almost any (high and/or low throughput) sequencing workflow required by our customers so that we optimize the quality and quantity of data output.
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- Consult with our experts for assistance with your research projects, request a quote!
- Review sample submission guidelines and instructions
- Doski, Shadin, et al. “Complete sequence and genome characterization of a new potexvirus isolated from Chaenostoma cordatum.” Archives of Virology 167.10 (2022): 2089-2092.
- Cervantes, Jorge, et al. “Investigation of oral, gastric, and duodenal microbiota in patients with upper gastrointestinal symptoms.” Journal of Investigative Medicine 69.4 (2021): 870-877.
- Gavrili, Vasileia, et al. “First report of barley virus G infecting corn in Greece.” Journal of Plant Pathology 103 (2021): 1331-1331.
- Leung, Marcus HY, et al. “Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants.” Microbiome 8.1 (2020): 1-17.
- Wintermantel, W. M., et al. “First report of Cucurbit chlorotic yellows virus infecting melon in the New World.” Plant Disease 103.4 (2019): 778-778.
Find other Research Publications from around the world!