Sample Input Recommendations

The following are SeqMatic’s recommendation for sample input for our library generation services. Please consult with your SeqMatic contact for assistance if your samples cannot meet our requirements.

Sample Cells or Tissues:

  • Please discuss with SeqMatic scientist regarding tissue quantity required
  • All samples must be flash frozen or stored in RNA Later

Genomic or Amplicon DNA:

  • Recommended Volume: 20uL or more
  • Recommended Concentration: 20 ng/uL or higher
  • Minimum Volume: 10uL
  • Minimum Concentration: 10 ng/uL
  • A260:A280 value should be between 1.8 and 2.0
  • DNA should be buffered in 10mM Tris-HCl or TE buffer
  • Amplicon DNA should be free of PCR primers and dimers

Purified RNA:

  • Recommended Amount: 1000ng or more
  • Recommended Volume: 20uL or more
  • Recommended Concentration: 20 ng/uL or higher
  • Minimum Amount: 100ng (if less than 100ng RNA is avaialbe, discuss with SeqMatic scientist prior to sample submission)
  • Minimum Volume: 10uL
  • Minimum Concentration: 5 ng/uL (if less than 5 ng/uL, discuss with SeqMatic scientist prior to sample submission)
  • A260:A280 value should be between 1.8 and 2.0
  • RNA should be stored in nuclease free water or RNAstable.
  • RNA must be validated using an assay such as a BioAnalyzer RNA chip. RNA QC services are available for a fee.

Prepared Sequencing Library (MiSeq & NextSeq):

  • Recommended Volume: 20uL or more
  • Recommended Concentration: 10nM or higher
  • Minimum Volume: 10uL
  • Minimum Concentration: 2nM
  • Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
  • Provide at least 10uL of 100uM custom sequencing primers, if required

Prepared Sequencing Library (NovaSeq):

S1 S2 S4
Recommended Volume: 200+ uL 200+ uL 200+ uL
Recommended Concentration: 10+ nM 10+ nM 10+ nM
Minimum Volume: 100uL 150uL 310uL
Minimum Concentration: 1nM 1nM 1nM
  • Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
  • Provide at least 20uL of 100uM custom sequencing primers, if required