REVIO - Long-read Sequencing Services
LONG-READ NEXT GENERATION SEQUENCING AT SCALE
REVIO long-read sequencing services help you achieve more with less DNA input. HiFi reads combine long-read length with high accuracy from parallel sequencing of four independent stages, and it is fully automated.
The PacBio REVIO system utilizes nanofabricated Revio SMRT® Cells, each of which has 25 million zero-mode waveguide wells.
The REVIO HiFi sequencing system best utility is for genome assembly, DNA methylation, RNA methylation, structural variation detection, and long-read transcriptome analysis with uniform coverage.
Inquire about SeqMatic’s long-read sequencing solutions. An expert can help you optimize your workflow goals with system recommendations catered to your analysis needs.
Service Highlights
Key Applications & Sample Throughput with SPRQ chemistry
Service Highlights
 | PacBio REVIO Long-read Sequencing ServicesHIFI Level Accuracy |
Long-read Sequencing Service Highlights |
- Expert Consultation – Our scientists can help you select the most scientifically viable and cost-effective parameter choices for your run design, sample load and barcode assignment requirements
- Comprehensive Services – Complete DNA workflow, including extraction from virtually any sample type, library generation, sequencing, and data analysis services
- Prepare Libraries From Difficult Samples – Low input DNA (as low as 1ng), blood, blood cards, saliva, stool, cell-free DNA and FFPE
- Stringent Quality Controls – All sample preparation and library construction steps are validated by multiple assays (Qubit, TapeStation, qPCR and test sequencing)
- Fast Turnaround Time – Standard and Express Services available
- Technical Support – QC reports prepared for review by the customer to decide which samples qualify for library preparation, or alternative library preparation/sample re-submission
- Flexibility – Choice for sample processing: manually or automated using a wide-range of processing technologies
- Wide-Range of Sample Types – human, animal, fish, from virtually any source
Key Applications & Sample Throughput with SPRQ chemistry
The Revio system supports a variety of applications that benefit from accurate long HiFi reads and requires only 500 ng of input DNA per sample.
| Library | Application | Per Revio SMRT Cell | Per Year* | Expected Coverage† |
| 0.5–5 kb | Amplicon sequencing | >1,000 | >2.5 million | 50× |
| 5–10 kb | Microbial genome | 384 | 480,000 | 30× |
| 5–15 kb | PureTarget panels | 96 | 96,000 | >100× mean per target |
| 5–10 kb | Ampli-Fi human genome | 1 | 1,2002 | 20× |
| 15–20 kb | Human genome | 2 | 2,500 | 20× |
| 15–20 kb | Human methylation profiling | 8 | 10,000 | 5× |
| 15–20 kb | Transcriptome with Kinnex™ full-length RNA kit | 6 | 7,500 | 10 million reads |
*Annual throughput is based on 2,500 Revio SMRT Cells for 12 hour runs; 1,250 for 24 hour runs; and 1,050 for 30 hour runs. Adaptive loading — which increases the
consistency of yield per SMRT Cell — adds about 4 hours to run time, affecting the maximum number of SMRT Cells per year.
† Expected coverages are estimates.
