Chromium X Series
Single Cell RNA Sequencing Services
10x Genomics Certified Service Provider
10x Chromium Single-Cell services for scientific research with high gene sensitivity and increased sample recovery. SeqMatic is a 10x Genomics certified service provider (CSP) laboratory for single cell gene expression (Flex and Universal 3′ or 5′), immune profiling BCR and/or TCR, Epi Chromatin (ATAC-seq), and Epi multiome gene expression. We offer Chromium X series systems for single cell workflow sequencing needs.
10x Chromium Single-Cell Services Highlights
Cell Sequencing Library Types
- Single Cell Gene Expression (Flex and Universal 3′ or 5′)
- Immune Profiling (BCR and/or TCR)
- Epi Chromatin (ATAC-Seq)
- Epi Multiome (ATAC + GEx)
Project Design Solutions Based On
- Cell/Tissue type (whole cell or
disassociated nuclei) - Cell conditions (fresh or cryopreserved)
- Number of cells per sample
- Sequencing read depth per cell
- BioInformatic requirements
- Other specialized assays
- Onsite-Seq™* and Fetch-Seq™*
We can process full workflows or accept your fresh cells, GEMs, cDNAs or prepared libraries.
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 | 10x Genomics
20K cells (1-8 samples) using Chromium Universal 3′ or 5′ kit Up to 1M cells (1-384 samples) using Chromium Flex Apex kit
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Comprehensive Chromium X series systems Services for single cell workflow sequencing needs.
Single cell gene expression (Flex and Universal 3′ or 5′), immune profiling BCR and/or TCR, Epi Chromatin (ATAC-seq), and Epi multiome gene expression.
Expert Volume Handling
From small projects with a few samples or low input to large scale high-throughput projects
Wide Range of Sample Types
- Human and mouse for Universal 3′, 5′ or Flex Apex
- Other animals, plant, fish, etc. for Universal 3′ and 5′ only
- Fresh/frozen cells and tissue or FFPE samples
Bioinformatics
- Full suite of custom and 10x Genomics analytic pipelines (Cell Ranger) available for individual project needs
- Additional analysis available such as cell annotation, differential expression, doublet removal, batch correction, etc.
Sequencing Platforms
Sequencing on NovaSeq 6000 and NovaSeq X Plus
Stringent Quality Controls
Your single cell submissions are assessed with Countess III FL to quantify cell concentration in suspension and cell viability before Chromium processing. Working carefully with your SeqMatic team to prepare your submission ensures superior cell recovery and GEM formation.
Scientific considerations for read depth
SeqMatic recommends a read depth of 10 to 20K read pairs per cell/nucleus. Higher and lower read depths are available depending on model organism requirements and/or project needs.
Preliminary Sequencing
After library preparation, SeqMatic prepared libraries are pooled to customer requirements and the final QC is checked via preliminary sequencing. For Universal 3′ or 5′, libraries are then normalized and re-pooled based on the cell/nuclei count from the preliminary sequencing dataset.
World-class validation standard & practices
SeqMatic is equipped to ensure accurate, repeatable and reproducible results. SeqMatic’s quality program has been audited and approved as part of becoming a Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) certified sequencing facility. Our CLIA quality program requires the use of well-defined protocols to maintain the consistency and reproducibility of our NGS workflows.
Custom assay services
CITE-seq add-on services available for surface protein analysis. We offer a choice of single antibody to comprehensive panels from BioLegend and Proteintech.
 | Your S.F. Bay Area Advantage! To maintain maximum sample viability, your samples can be transported to us |
 | ∗ SeqMatic’s Premium Services |
- Ding, Xia, et al. “PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib.” Scientific Reports 12.1 (2022): 1-19.
- Lim, Yoong Wearn, et al. “Single-cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironment.” BMC biology 19.1 (2021): 1-17.
Find other Research Publications from around the world!
| Single Cell Gene Expression Certification  | Single Cell Immune Profiling Certification |
