Sample Input Recommendations
Services For Precious Samples
WE ACCEPT SAMPLES AT ANY STAGE OF THE PIPELINE
Sample input recommendations services are available to maximize your project workflow and the results for your sequencing projects. Above all, these recommendations help maintain sample integrity, ensure traceability, and enable workflow efficiencies in the laboratory.
In effect, following these sample input recommendations will help ensure that the data generated from laboratory analyses is accurate, reliable, and reproducible. This is fundamental for advancing your scientific research and making informed decisions based on the results.
The following are SeqMatic’s recommendation for sample input for our library generation services. Please consult with your SeqMatic contact for assistance if your samples cannot meet our requirements.
HIDDEN
HIDDEN
Sample Cells or Tissues:
- Please discuss with SeqMatic scientist regarding tissue quantity required
- We accept samples in a variety of formats, please contact SeqMatic for more information
- For RNA, samples can be flash frozen or stored in a RNA preservation solution such as RNA Later
Genomic or Amplicon DNA:
- Recommended Volume: 20uL or more
- Recommended Concentration: 20 ng/uL or higher
- Minimum Volume: 10uL
- Minimum Concentration: 10 ng/uL
- A260:A280 value should be between 1.8 and 2.0
- DNA should be buffered in 10mM Tris-HCl or TE buffer
- Amplicon DNA should be free of PCR primers and dimers
Purified RNA:
- Recommended Amount: 1000ng or more
- Recommended Volume: 20uL or more
- Recommended Concentration: 20 ng/uL or higher
- Minimum Amount: 100ng (if less than 100ng RNA is available, discuss with SeqMatic scientist prior to sample submission)
- Minimum Volume: 10uL
- Minimum Concentration: 5 ng/uL (if less than 5 ng/uL, discuss with SeqMatic scientist prior to sample submission)
- A260:A280 value should be between 1.8 and 2.0
- RNA should be stored in nuclease free water or RNAstable.
- RNA must be validated using an assay such as a BioAnalyzer RNA chip. RNA QC services are available for a fee.
Prepared Sequencing Library (MiSeq & NextSeq):
- Recommended Volume: 20uL or more
- Recommended Concentration: 10nM or higher
- Minimum Volume: 10uL
- Minimum Concentration: 2nM
- Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
- Provide at least 10uL of 100uM custom sequencing primers, if required
Prepared Sequencing Library (NovaSeq):
SP/S1 | S2 | S4 | |
Recommended Volume: | 200+ uL | 250+ uL | 350+ uL |
Recommended Concentration: | 10+ nM | 10+ nM | 10+ nM |
Minimum Volume: | 100uL | 200uL | 300uL |
Minimum Concentration: | 1nM | 1nM | 1nM |
- Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
- Provide at least 20uL of 100uM custom sequencing primers, if required
Prepared Sequencing Library (NovaSeq Xp Lane Loading):
SP/S1 | S2 | S4 | |
Recommended Volume: | 50+ uL | 50+ uL | 60+ uL |
Recommended Concentration: | 10+ nM | 10+ nM | 10+ nM |
Minimum Volume: | 20uL | 30uL | 40uL |
Minimum Concentration: | 1nM | 1nM | 1nM |
- Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
- Provide at least 20uL of 100uM custom sequencing primers, if required

The SeqMatic Difference
Expert NGS
The SeqMatic approach starts with proven genomic workflows and proprietary controls that have been refined and optimized over the past decade to deliver considerably more data than Illumina specifications.
Expert NGS
The SeqMatic approach starts with proven genomic workflows and proprietary controls that have been refined and optimized over the past decade to deliver considerably more data than Illumina specifications.
View MoreConscientious Attention To Detail
We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.
Conscientious Attention To Detail
We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.
View MoreTailor-made Services with Fast TAT
Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.
Tailor-made Services with Fast TAT
Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.
View MoreOptimal Solutions to Research Needs
This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.
Optimal Solutions to Research Needs
This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.
View MoreGet Your Results
Optimal solutions are custom-made according to research needs




Pre-Submission
Planning & Consultation - Assurance of de-identified sample submission and handling
Sample Submission
Nucleic Acid Extraction - We accept a wide range of sample types
Library Preparation
Library Generation - Amplified cDNA is used for sequence libraries' construction
Sequencing
Different platforms for different data outputs
Data Analysis
Custom BioInformatics