Sample Input Recommendations
Services For Precious Samples

WE ACCEPT SAMPLES AT ANY STAGE OF THE PIPELINE

Sample input recommendations services are available to maximize your project workflow and the results for your sequencing projects. Above all, these recommendations help maintain sample integrity, ensure traceability, and enable workflow efficiencies in the laboratory.

In effect, following these sample input recommendations will help ensure that the data generated from laboratory analyses is accurate, reliable, and reproducible. This is fundamental for advancing your scientific research and making informed decisions based on the results.

The following are SeqMatic’s recommendation for sample input for our library generation services. Please consult with your SeqMatic contact for assistance if your samples cannot meet our requirements.

HIDDEN

Sample Cells or Tissues:

Please discuss with SeqMatic scientist regarding tissue quantity required
We accept samples in a variety of formats, please contact SeqMatic for more information
For RNA, samples can be flash frozen or stored in a RNA preservation solution such as RNA Later

Genomic or Amplicon DNA:

Recommended Volume: 20uL or more
Recommended Concentration: 20 ng/uL or higher
Minimum Volume: 10uL
Minimum Concentration: 10 ng/uL
A260:A280 value should be between 1.8 and 2.0
DNA should be buffered in 10mM Tris-HCl or TE buffer
Amplicon DNA should be free of PCR primers and dimers

Purified RNA:

Recommended Amount: 1000ng or more
Recommended Volume: 20uL or more
Recommended Concentration: 20 ng/uL or higher
Minimum Amount: 100ng (if less than 100ng RNA is available, discuss with SeqMatic scientist prior to sample submission)
Minimum Volume: 10uL
Minimum Concentration: 5 ng/uL (if less than 5 ng/uL, discuss with SeqMatic scientist prior to sample submission)
A260:A280 value should be between 1.8 and 2.0
RNA should be stored in nuclease free water or RNAstable.
RNA must be validated using an assay such as a BioAnalyzer RNA chip. RNA QC services are available for a fee.

Prepared Sequencing Library (MiSeq & NextSeq):

Recommended Volume: 20uL or more
Recommended Concentration: 10nM or higher
Minimum Volume: 10uL
Minimum Concentration: 2nM
Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
Provide at least 10uL of 100uM custom sequencing primers, if required

Prepared Sequencing Library (NovaSeq):

	SP/S1	S2	S4
Recommended Volume:	200+ uL	250+ uL	350+ uL
Recommended Concentration:	10+ nM	10+ nM	10+ nM
Minimum Volume:	100uL	200uL	300uL
Minimum Concentration:	1nM	1nM	1nM

Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
Provide at least 20uL of 100uM custom sequencing primers, if required

Prepared Sequencing Library (NovaSeq Xp Lane Loading):

	SP/S1	S2	S4
Recommended Volume:	50+ uL	50+ uL	60+ uL
Recommended Concentration:	10+ nM	10+ nM	10+ nM
Minimum Volume:	20uL	30uL	40uL
Minimum Concentration:	1nM	1nM	1nM

Libraries should be pooled prior to submission. Library pooling services are available for an additional fee.
Provide at least 20uL of 100uM custom sequencing primers, if required

The SeqMatic Difference

Expert NGS

The SeqMatic approach starts with proven genomic workflows and proprietary controls that have been refined and optimized over the past decade to deliver considerably more data than Illumina specifications.

Expert NGS

Conscientious Attention To Detail

We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.

Conscientious Attention To Detail

We learn about the goals of our customer’s scientific research and the type of sequencing data that would be most beneficial to their project.

Tailor-made Services with Fast TAT

Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.

Tailor-made Services with Fast TAT

Then we modify our workflow to target those specific reads. Couple that effort with world class turnaround time.

Optimal Solutions to Research Needs

This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.

Optimal Solutions to Research Needs

This application of best science, higher quality data, more read depth per lane, and fast delivery makes SeqMatic an exceptional partner and value per research dollar spent.

Get Your Results

Optimal solutions are custom-made according to research needs

Pre-Submission

Planning & Consultation - Assurance of de-identified sample submission and handling

Sample Submission

Nucleic Acid Extraction - We accept a wide range of sample types

Library Preparation

Library Generation - Amplified cDNA is used for sequence libraries' construction

Sequencing

Different platforms for different data outputs

Data Analysis

Custom BioInformatics

Get a Quote

Customer Resources