Histopathology Staining Assay Services

Advancing tissue-based research with white-glove staining capabilities

Histopathology staining assay services enable pathologists to evaluate tissue samples, diagnose conditions, and interpret findings through microscopy. The selection of stains is contingent upon the investigational question.

At SeqMatic, the histopathology staining assay services offer routine staining (H&E), chromogenic immunohistochemistry (IHC) and fluorescent staining, and special stain services for high level detail analysis.

HIDDEN

Hematoxylin & Eosin (H&E)

CONSISTENT & REPEATABLE STAINING QUALITY

The Hematoxylin and Eosin (H&E) staining is the standard and preferred method for viewing cellular and tissue structure detail of routine diagnostics. This stain method is simple, fast, stable, and affordable.

Immunohistochemistry (IHC)

VISUALIZING ANTIBODY-ANTIGENS

The chromogenic immunohistochemistry (IHC) and fluorescent staining enables selective identification of cell antigens in tissue sections. SeqMatic’s immunohistochemical staining is used for projects that involve the visualization of abnormal cells in oncology research projects; as well as analysis of the distribution and localization of biomarkers and differentially expressed proteins in different parts of biological tissue.

Visualization of antibody-antigen interaction is achieved via:

- Chromogenic immunohistochemistry (CIH) – antibodies are used to detect antigens in tissue using a reporter enzyme and a colored chomogen
- Immunofluorescence – where the antibody is tagged to a fluorophore; SeqMatic offers 4 channels FITC, TRITC, CY5 and DAPI

Other Special Stains

ADDITIONAL CUSTOM DETAIL FOR RESEARCH ANALYSIS

Special stain services provide a high level of detail for analyzing tissues such as kidney, liver, lung, heart, spleen and skin. These stains include Trichrome Masson, Alcian Blue pH 2.5, Oil Red O, and Von Kossa. Through these services precise analysis is possible, allowing for the visualization and identification of specific cells, tissue types, and cellular products.

Adenonocarcinoma from human breast cancer, ductal cell carcinoma, tissue section by H and E stain
Cytotoxic T-Cells Marker: CD8a Tissue: Tonsil RUO Validation
M1 Macrophages Marker: CD68 Tissue: Tonsil RUO Validation
Kidney 10x
Leukocytes Marker: CD45 Tissue: Tonsil RUO Validation
pan T-Cells Marker: CD3 Tissue: Tonsil RUO Validation
Proliferation Marker: Ki-67 Tissue: Tonsil Localization: Nuclear RUO Validation
Pig Liver HE 3o5x
M2 Macrophages Marker: CD163 Tissue: Tonsil RUO Validation
Helper T-Cells Marker: CD4 Tissue: Tonsil RUO Validation
Immune checkpoint protein Marker: PD-L1 Tissue: Tonsil RUO Validation